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mreC [2018-02-13T14:18:20.000Z]

MreC is a cell shape determining protein, it couples the cytosolic MreB and MreB-like proteins to the extracellular peptidoglycan-synthesizing machinery

locus

BSU28020

6.25

32.00 kDa

protein length

290 aa Sequence Blast

gene length

870 bp Sequence Blast

function

cell shape determation

product

cell shape-determining protein

essential

yes

synonyms

Outlinks

KEGG BsubCyc SubtiList UniProt Expression Browser

Genomic Context

Hide small RNAs Zoom:

categories

[category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.2|Cell shape]

[category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]

[category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

[category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates

2,859,832 → 2,860,704

Phenotypes of a mutant

''mreC'' is essential under normal conditions [Pubmed|12682299].

Depletion of MreC leads to a progressive increase in the width and a decrease in the length of the cell. This shape defect is consistent with a role for ''mreC'' in cell wall synthesis during elongation and has a similar phenotype to other genes with roles in elongation like ''[gene|B405B3C21B464F904BBB2AFD5DA21ADE45B4DD96|rodA]'' and the redundant gene pair ''[gene|3B4F035535D6504405567E7C44E72902A11F7447|pbpA]'' and ''[gene|883DFF72888D280A102E53CC1D69A8B1C7BE2907|pbpH]''.

Electron microscopy of cells depleted of MreC shows regions of the cell where a thick and irregular cell wall has accumulated [Pubmed|12867458,16101995].

''mreC'' can be deleted provided that 0.5 M sucrose and 20 mM Mg(2 ) is provided in the media, ''mreC'' is therefore conditionally essential. The phenotype of the ''mreC'' deletion in these conditions is one characterised by extreamly fat and bloated cells that tend to grow in clusters [Pubmed|16101995].

The protein

Catalyzed reaction/ biological activity

None/ structural protein

Protein family

mreC family (according to Swiss-Prot) COG1793

Paralogous protein(s)

None

[SW|Domains]

Intracellular N-terminus, transmembrane domain, Coiled coil domain and C-terminal beta-sheet domain.

Structure

[PDB|2J5U]: MreC from ''Listeria monocytogenes'' [Pubmed|17427287]

[PDB|2QF4]: MreC monomer from ''Streptococcus pneumoniae'' [Pubmed|17707860]

[PDB|2QF5]: MreC dimer from ''Streptococcus pneumoniae'' [Pubmed|17707860]

[SW|Localization]

trans-membrane protein [Pubmed|21636744,16101995]

during logarithmic growth, [protein|231C30963AFB863424C087CB722446D319976D65|MreD] forms discrete patches thst move processively along peripheral tracks perpendicular to the cell axis [Pubmed|21636744]

forms transverse bands as cells enter the stationary phase [Pubmed|21636744]

reports on helical structures formed by MreC [Pubmed|16101995,20566861] seem to be misinterpretation of data [Pubmed|21636744]

Expression and Regulation

Operons

genes

description

[Pubmed|8459776]

sigma factors

regulatory mechanism

regulation

constitutively expressed [Pubmed|23701187]

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genes

description

[Pubmed|22383849]

sigma factors

regulatory mechanism

regulation

constitutively expressed [Pubmed|23701187]

view in new tab

Biological materials

Mutant

A non-polar inframe deletion strain named 3481 and a xylose dependent conditional mutant named 3461 is avaliable from the [SW|Errington] lab [Pubmed|16101995].

GFP fusion

A functional N-terminal GFP fusion has been made where the fusion protein is the only copy of the gene in the cell: strain 3417 [Pubmed|16101995].

Antibody

antisera raised in rabit is avaliable from the [SW|Errington] lab.

Labs working on this gene/protein

[SW|Jeff Errington], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington homepage]

[SW|Peter Graumann], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]

References

20566861,21636744,21636745,18363795,17427287,18179421,8459776,21926231,16101995,22383849,26091431